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h blocking step  (Proteintech)


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    Structured Review

    Proteintech h blocking step
    H Blocking Step, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+blocking+step/PTPN5+Antibody/pm40921293-83-2-36
    Average 96 stars, based on 135 article reviews
    h blocking step - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma
    Article Snippet: The proteins were then isolated by electrophoresis and transferred to a polyvinylidene fluoride (PVDF) membrane (Invitrogen, USA). .. Following a 2-h blocking step with 5% skim milk, incubation with PRELP antibody (1:1,000, Proteintech, China) was conducted overnight at 4°C. .. The membrane was washed and incubated with the corresponding secondary antibody (1:1,000, Proteintech, China).

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2-AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Article Title: STING modulates iron metabolism to promote liver injury and inflammation in acute immune hepatitis.
    Article Snippet: The pathogenesis of Autoimmune Hepatitis (AIH) is closely associated with perturbations in iron ion metabolism, during which Stimulator of Interferon Genes (STING) plays an important role.. However, the precise regulatory mechanism remains elusive.. In this study, we investigated the relationship between iron dysregulation and STING activation in Concanavalin A (ConA)-induced AIH liver injury.

    Article Title: Period circadian regulator 2-mediated steroid hormone synthesis by regulating transcription of steroidogenic acute regulatory protein in porcine granulosa cells.
    Article Snippet: Steroidogenesis is associated with circadian clock genes.. However, the regulation of steroid hormone production in sow granulosal cells by Per2, a crucial circadian regulator, remains unexplored.. In this study, we have identified the presence of Per2 in ovarian granulosa cells and have observed its circadian expression pattern.

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature.
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2- AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Article Title: Dysregulation of SRSF3/ circSAMD4 /CIRBP Axis Promotes Iodinated Contrast-induced Acute Kidney Injury
    Article Snippet: .. After a 1-h blocking step at room temperature, cells were incubated overnight at 4 °C with primary antibodies against CIRBP (10209-2-AP, Proteintech, Wuhan, China) or Cytokeratin 18 (A01357-1, Boster, Wuhan, China). .. The cells were then incubated with secondary antibodies - either Alexa-Fluor 488 (ab150077, Abcam, Cambridge, UK) or CoraLite594 (SA00013-4, Proteintech, Wuhan, China) - for 1 h at 37 °C.

    Article Title: T-2 toxin induces cardiotoxicity by activating ferroptosis and inhibiting heme oxygenase-1.
    Article Snippet: • T-2 toxin induced cardiac injury in mice and human cardiomyocytes.. • T-2 toxin activated cardiac ferroptosis in

    Incubation:

    Article Title: Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma
    Article Snippet: The proteins were then isolated by electrophoresis and transferred to a polyvinylidene fluoride (PVDF) membrane (Invitrogen, USA). .. Following a 2-h blocking step with 5% skim milk, incubation with PRELP antibody (1:1,000, Proteintech, China) was conducted overnight at 4°C. .. The membrane was washed and incubated with the corresponding secondary antibody (1:1,000, Proteintech, China).

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2-AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Article Title: STING modulates iron metabolism to promote liver injury and inflammation in acute immune hepatitis.
    Article Snippet: The pathogenesis of Autoimmune Hepatitis (AIH) is closely associated with perturbations in iron ion metabolism, during which Stimulator of Interferon Genes (STING) plays an important role.. However, the precise regulatory mechanism remains elusive.. In this study, we investigated the relationship between iron dysregulation and STING activation in Concanavalin A (ConA)-induced AIH liver injury.

    Article Title: Period circadian regulator 2-mediated steroid hormone synthesis by regulating transcription of steroidogenic acute regulatory protein in porcine granulosa cells.
    Article Snippet: Steroidogenesis is associated with circadian clock genes.. However, the regulation of steroid hormone production in sow granulosal cells by Per2, a crucial circadian regulator, remains unexplored.. In this study, we have identified the presence of Per2 in ovarian granulosa cells and have observed its circadian expression pattern.

    Article Title: Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature.
    Article Snippet: .. After a 1-h blocking step at room temperature, primary antibodies targeting human FASN, GLI2, PDGFRA, VHL or β-Actin from Proteintech Group, Wuhan, China, were incubated at 4 °C for 12 h. FASN (Cat. No. 10624-2- AP), GLI2 (Cat. No. 18989-1-AP) and β-Actin (Cat. No. 81115-1-RR) were purchased from Proteintech Group (Wuhan, China). .. PDGFRA (Cat. No. ab203491) were purchased from Abcam (Cambridge, UK).

    Article Title: Dysregulation of SRSF3/ circSAMD4 /CIRBP Axis Promotes Iodinated Contrast-induced Acute Kidney Injury
    Article Snippet: .. After a 1-h blocking step at room temperature, cells were incubated overnight at 4 °C with primary antibodies against CIRBP (10209-2-AP, Proteintech, Wuhan, China) or Cytokeratin 18 (A01357-1, Boster, Wuhan, China). .. The cells were then incubated with secondary antibodies - either Alexa-Fluor 488 (ab150077, Abcam, Cambridge, UK) or CoraLite594 (SA00013-4, Proteintech, Wuhan, China) - for 1 h at 37 °C.

    Article Title: T-2 toxin induces cardiotoxicity by activating ferroptosis and inhibiting heme oxygenase-1.
    Article Snippet: • T-2 toxin induced cardiac injury in mice and human cardiomyocytes.. • T-2 toxin activated cardiac ferroptosis in

    Staining:

    Article Title: STING modulates iron metabolism to promote liver injury and inflammation in acute immune hepatitis.
    Article Snippet: The pathogenesis of Autoimmune Hepatitis (AIH) is closely associated with perturbations in iron ion metabolism, during which Stimulator of Interferon Genes (STING) plays an important role.. However, the precise regulatory mechanism remains elusive.. In this study, we investigated the relationship between iron dysregulation and STING activation in Concanavalin A (ConA)-induced AIH liver injury.

    Saline:

    Article Title: LncRNA HANR promotes the aerobic glycolysis in prostate cancer by stabilizing TPI1.
    Article Snippet: Prostate cancer (PCa) is a type of malignancy that originates in the prostate gland, often characterized by uncontrolled cell growth and potential metastasis.. Long non-coding RNAs (lncRNAs) play crucial regulatory roles in the progression of prostate cancer, potentially facilitating tumor growth and metastasis via mechanisms that involve the enhancement of aerobic glycolysis.. This study aimed to investigate the functional role of lncRNA HANR in prostate cancer progression.

    Membrane:

    Article Title: LncRNA HANR promotes the aerobic glycolysis in prostate cancer by stabilizing TPI1.
    Article Snippet: Prostate cancer (PCa) is a type of malignancy that originates in the prostate gland, often characterized by uncontrolled cell growth and potential metastasis.. Long non-coding RNAs (lncRNAs) play crucial regulatory roles in the progression of prostate cancer, potentially facilitating tumor growth and metastasis via mechanisms that involve the enhancement of aerobic glycolysis.. This study aimed to investigate the functional role of lncRNA HANR in prostate cancer progression.



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